Application

Detect SCN8A Sodium Channel using this Anti-SCN8A Sodium Channel Antibody validated for use in IC, IH & WB.

Western blot: (1:200) using ECL on rat brain membranes.

Immunohistochemistry on fixed frozen sections.

Immunocytochemistry on rat dorsal root ganglion primary culture (1:100)

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

General description

Voltage-gated sodium channels perform critical roles for electrical signaling in the nervous ssystem by generating action potentials in axons and dndrites. At least 10 genes encode sodium channels in mammals, but specific physiological roles that distinguish each of these isoforms are not well known. Nav1.6 is highly concentrated at notes of Ranvier of both sensory and motor axons in the peripheral nervous system and at nodes in the central nervous system. Because of its consistant and restrictied labeling, Anti-Nav1.6 (AB5580) has been used effectively as a nodes of Ranvier marker.

Immunogen

Peptide corresponding to amino acids 1042-1061 from mouse (Accession number AAD20438) or rat (Accession number AAC26014) Scn8a.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Specificity

Recognizes Scn8a (Nav1.6). The epitope corresponds to the intracellular loop between II and III domains of Scn8a. Does not cross react with any other known proteins.

SPECIES REACTIVITIES: It is expected that the antibody will also work on mouse (20/20) and human (19/20) due to sequence homology. Other species have not been tested.